The present invention refers to a novel molecularly imprinted polymer (MIP) obtainable by polymerization of monomers in the presence of a template molecule barbituric acid (BA). The obtained linear MIP may be used for selective separation of the template analyte from blood samples in aqueous samples, effluents, pharmaceutical preparations in the presence of other structurally related interferents. Present invention further lad refers to the preparation of new a silica gel bound molecularly imprinted polymer-based sorbent of column chromatography for the selective recognition of barbituric acid (BA). The optimized operational conditions for 100% retention of the barbituric acid on a chromatographic column (13.0 X 1.4 cm), packed with adsorbent bed 1.0 g, is realized at pH 7.0 and a flow rate of 5.0 mL min-1. The MIP-based adsorbent can be instantaneously regenerated for reuse after washing with hot water within 15 min.