: PRODUCTION PROCESS OF RECOMBINANT PLACENTAL GROWTH FACTOR
Application 1130/KOLNP/2004 published 2006-04-28, filed 2004-08-09
Process for extracting and purifying the recombinant Placental Growth Factor (PLGF) expressed in inducible prokaryotic expression systems comprising the following steps: I) fermentation of the bacterial cells, II) extraction and purification of the inclusion bodies, III) renaturation of the expressed protein, IV) ion-exchange chromatography, V) reverse-phase chromatography. (FIG.1)
Applicant
GEYMONAT S.P.A.
Via S. Anna, 2, I-03012 Anagni, ITALY.
Inventor
MAGLIONE, Domenico BATTISTI, Mauro CONTI, Ettore SALVIA, Giuseppe TUCCI, Marina
International Info
Classification: C07K 14/475, C12N 15/12
Publication Number: WO 03/066676
Application Date: 2002-02-05