BigPatents India

A process for preparation of phosphate solubilizing liquid bio-fertilizer comprising the steps of isolating and purifying Aspergillus awamori from soil by standard isolation procedures on Pikovskaya’s agar medium plates; carrying out solid state fermentation by inoculating spores on modified Pikovskaya’s agar medium plates; incubating at room temperature for 7 days; preparing spore suspension by adding 1 ml tween-80, 1ml glycerol, 15ml sterile saline to plate of modified Pikovskaya’s agar medium plate incubated for said period and mixing; inoculating spore suspension using cotton bungs wrapped in cotton gauze on Pikovskaya’s agar medium plates; incubating the inoculated plates at room temperature for 7 days; blending and homogenizing 40% mycelial growth along with spores; 10% glycerol; 2% tween-80 at 1100 rpm using a high speed mixer; adding 0.1 % streptocyclin to the blended formulation and sterile water Q.S to make 100%. Drawing: NIL Total Pages: 11 Fig. Nil