BigPatents India

A process for extracting 3-(bb glucosido) indole comprising obtaining plant/plant part containing 3-(bb glucosido) indole, inactivating the plant enzyme, indimulsin in the plant/plant part in a manner as herein described for preventing degradation of 3-(b glucosido) indole and extracting the 3-(bb glucosido) indole into the broth, treating the broth, treating the broth with an alkali to adjust the ph of the said broth to a value between 7 to 11, clarifying and sterilizing the broth in a manner as herein described, passing the sterilized clarified broth through a column/bed of sterilized anion exchange resin with exchangeable hydroxyl or chloride for removing the anions, passing the sterilized broth through a column bed of sterilized column bed cation exchange resin with exchangeable hydronium or alkali cation, concentrating the de-ionized 3-(bb glucosido) indole by passing it over sterile absorbent column, eluting 3-(bb glucosido) indole from the absorbent column with organic solvents and recovering the said organic solvent by distillation or evaporation. Drawing Sheets. NIL Total Pages: 09 (FIG.-NIL)