BigPatents India

A method for designing the process for microsatellite region of piper nigum comprising: isolating DNA from the plant, subjecting the said DNA to the step of digestion, adding an adapter to both ends of the DNA fragments; Amplifying the said adapter ligated DNA using Tsp primers; subjecting the amplified DNA to the step of hybridization, enriching the hybridization DNA fragments for microsatellite repeats; subjecting the enriched DNA fragments to the step of tailing to make the ligation of the fragments into pGEMT vector; cloning into pGEMT vector to select transformed colonies; sequencing the clones to design primers complementary to the flanking regions of the microsatellite repeats.