METHODS FOR MULTIPLE PARAMETER SCREENING AND EVOLUTION OF CELLS TO PRODUCE SMALL MOLECULES WITH MULTIPLE FUNCTIONALITIES.
Application 1873/CHENP/2004 published 2006-06-23, filed 2004-08-23
The present invention relates to the field of selecting cells producing compounds that fulfil at least 2 predetermined functionalities. The invention primarily focuses on methods for screening of cells for two or more functionalities related to small molecules generated by host cells with novel gene assemblies. The methods in one aspect of the invention are combined with methods for evolution of cells to acquire at least two predetermined functionalities related to small molecules. Furthermore, the invention relates to methods for generation of lead compounds that are selected for a primary function and at least one further secondary function. The methods according to the invention may in particular be used in high troughput screening.
Applicant
EVOLVA LTD ,
Hangmattstasse 6, 4123 Allschwil, Switzerland
Inventor
International Info
Classification: C12N 15/10
Publication Number: WO 03/062419
Priority Information
PA 2002 01174 . PCT/DK02/00057 . DENMARK 2002-08-01