M.tb ISOCITRATE DEHYDROGENASES DISPLAY STRONG B-CELL RESPONSE AND DIFFERENTIATE BCG-VACCINATED HEALTHY CONTROLS FROM TB PATIENTS.
Application 710/CHE/2004 published 2006-06-16, filed 2004-07-21
Proteins that are released from Mycobacterium tuberculosis during late logarithmic growth phase are often considered as candidate components of immunogenic or autolysis markers. One such protein is isocitrate dehydrogenase (ICD), a key regulatory enzyme in the citric acid cycle. We have evaluated the immunogenic properties of the two isoforms of M. tuberculosis ICDs and compared the same with the control antigen -HSP 60 as well as purified protein derivative (PPD). PPD lacks the sensitivity to distinguish between BCG vaccinated and TB-infected populations and therefore epidemiological relevance of PPD in BCG vaccinated regions is debatable. We show that M.tb ICDs elicit a strong B-cell response in TB- infected population and can differentiate between healthy-BCG vaccinated population and those with tuberculosis. The study population (n=215) was categorized into different groups, namely, patients with fresh infection (n=42), relapsed TB cases (n=32), patients with extra- pulmonary tuberculosis (n=35), clinically healthy donors (n=44), NTMs (n=30) and non-TB patients (culture negative for acid fast bacteria but carrying other infections, n=32). The M.tb ICDs showed statistically significant antigenic distinction between healthy-BCG vaccillated controls and tuberculosis patients (p
Applicant
CENTRE FOR DNA FINGERPRINTING AND DIAGNOSTICS
DEPARTMENT OF BIOTECHNOLOGY,, MINISTRY OF SCIENCE & TECHNOLOGY,GOVERNMENT OF INDIA,, NACHARAM,HYDERABAD,500 076,, INDIA.
Inventor
International Info
Classification: A 61 K 49/00 , C 12 N 9/04
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