BigPatents India

The present invention relates to a method of isolating liver stem cells, said method comprising steps suspending the hepatocytes in Oulbecco's Minimum Essential Medium (OM EM) with about 10% Fetal calf serum, centrifuging the suspended cells, obtaining liver stem cells as a last fraction in the pump flow rate ranging between 35-40 ml/minute; also, an in vitro method of trans-differentiating liver stem cells into pancreatic cell under specific culture conditions; in addition, a method of treating diabetes Type I in a subject, said method comprising steps of transplanting transdifferentiated (TO) pancreatic progenitors cells intrahepatically in the subject, and obtaining the subject with increased levels of insulin, and normalized glucose levels; further, a method of liver transplantation in a subject using liver stem cells, said method comprising steps of injecting about 10 million mammalian fetal hepatic stem cells intrahepatically into the subject, and obtaining transplanted liver of normal architecture.