BigPatents India

The invention relates to a process for the storage of culture, such as mycorrhizae, comprising in the steps of (i) selecting the fungus, namely Laccaria Laccata; (ii) introducing the fungus into a medium selected from potato dextrose agar or MMN and having a Ph of 7.0 to 7.5; (ii) allowing the fungus of age; (iv) subjecting the fungus to the step of pre-freezing in the presence of cryoprotectants such that the temperature of pre-freezing is maintained not above than – 30oC, (v) subjecting the fungus to the step of freeze drying by a tube in tube method at a temperature not above – 100oC. Further, the invention is related to a process for revitalizing freeze dried mycorrhizae which comprises in reconstituting the dried culture in a rehydrant for a period to reconstitute the culture, placing the disc containing the reconstituted culture on a MMN agar plate and subjecting it to the step of incubation.