"MODIFIED HEMOGLOBIN AND METHODS OF MAKING SAME"`
Application 2820/DELNP/2005 published 2007-01-19, filed 2005-06-24
The present invention provides a hemoglobin molecule (Hb) having six ± one PEG chains, wherein two of said PEG chains are bound to Cys-93 (6) of Hb, and the remaining PEG chains are bound to thiol groups introduced on E-NH2 of Hb. The present invention also provides a process for preparing a modified hemoglobin molecule (Hb), comprising the steps of: (a) reacting Hb with 8-15 fold excess of iminothiolane to form thiolated Hb; and (b) reacting the thiolated Hb with 16-30 fold excess of PEG functionalized with a maleimide moiety, to form the modified Hb.
Applicant
1)ALBERT EINSTEIN COLLEGE OF MEDICINE OF YESHIVA UNIVERSITY
:1300 MORRIS PARK AVENUE, BRONX, NY 10461, USA U.S.A.
Inventor
1)SEETHARAMA A. ACHARYA 2)BELUR N. MANJULA
International Info
Classification: A61K 38/16
Publication Number: WO 2004/058291
Application Date: 2003-12-18
Priority Information
60/436,149 U.S.A. 2002-12-23