BigPatents India

This invention relates to a sterilization method for the production of implantable or transplantable biological material of animal or human origin.The basic inventive idea is that the animal source material is treated in a combination of four successive procedural steps, each of which being effective in a specific time interval, first in an antibiotic solution, then in a deoxycholic acid solution, then in a surface-active substance, and finally in a primary alcohol. The sterilizing substance used in each step has both a physical, i.e. a separating and removing, and a chemical sterilization effect on the respective microorganisms. The second to fourth treatment steps are each followed by a rinsing process with an aqueous solution, preferably a sodium chloride solution, to remove the sterilizing agent and the separated microorganisms. It is important to perform the sterilization and rinsing steps in the specified sequence and in a specific time interval.