MICROBIAL 9- HYDROXYLATION OF STEROIDS
Application IN/PCT/2002/741/CHE published 2007-08-03, filed 2002-05-20
A method is described to construct genetically modified strains of steroid degrading micro-organisms wherein multiple genes involved in steroid nucleus degradation are inactivated, such as steroid dehydrogenase genes. Examples for such genes are kstD1 and kstD2: strains with multiple inactivated steroid degrading enzyme genes can be used in the accumulation of steroid intermediates with a high yield. A preferred accumulation product is 9-hydroxy-4-androstene-3,17-dione.
Applicant
1)AKZO NOBEL N.V.
:Velperweg 76 NL-6824 BM Arnhem U.S.A.
Inventor
1)AKZO NOBEL N.V.
International Info
Classification: C12N 9/02
Publication Number: WO/2001/031050
Application Date: 2001-05-03
Priority Information
99203470.2 Netherlands 1999-10-22