BigPatents India

A microbial culture having an enhanced glutaminase activity prepared by releasing the catabolite repression of a microorganism under incubation which is capable of producing glutaminase and, if required, supplying a nitrogen source at the medium stage of the incubation. A protein is treated with the microbial culture thus obtained in the presence of a protease under a salt-free condition or in the presence of not more than 3 % (weight/volume) of sodium chloride to thereby give a protein hydrolysat having a potent flavoring effect and being useful as a seasoning.