“ Enzymes involved in the triacylglycerol biosynthesis in the cytosol of Eukaryotes“
Application 472/DELNP/2004 published 2006-03-10, filed 2004-02-26
This invention describes novel catalytically active cytosolic enzymes for triacylglycerol biosynthesis from eukaryotic systems. The complex from oleaginous yeast was enzymatically characterized, and was found to contain lysophosphatidic acid acyltransferase, phosphatidic acid phosphatase, diacylglycerol acyltransferase, acyl-acyl carrier protein synthetase, superoxide dismutase and acyl carrier protein. The triacylglycerol biosynthetic machinery rapidly incorporates free fatty acids as well as fatty acyl-coenzyme A into triacylglycerol and its biosynthetic intermediates. Lysophosphatidic acid acyltransferase, phosphatidic acid phosphatase and diacylglycerol acyltransferase from the complex were microsequenced. Acyl carrier protein, superoxide dismutase and diacylglycerol acyltransferase genes were cloned and expressed in bacterial system. The soluble triacylglycerol biosynthetic enzymes (lysophosphatidic acid acyltransferase, phosphatidic acid phosphatase, diacylglycerol acyltransferase) in yeast, rat adipocytes and human hepatocyte cell-line (HepG2) exist in the cytosol either as free enzymes or as a multienzyme complex.
Applicant
1. Bijam Biosciences Limited 2. Indian Institute of Science
1. Plot No. 61, Nagarjuna Hills, Punjagutta Hills, 560 082, Hyderabad, India 2. Department of Biochemistry Malleswaram, 560 012, Bangalore, India
Inventor
RAJASHEKARAN, Ram
International Info
Classification: C12N 15/52
Publication Number: WO 03/018814
Application Date: 2002-08-29
Priority Information
60/315,757 US 2001-08-30