BigPatents India

The present invention provides a Micro-thermal cycler for DNA amplification comprising: DNA sample loading and unloading ports, a reaction chamber containing the appropriate primers and dNTP connected to said ports using micro-fluidic channels, said reaction chamber is provided with heaters and temperature sensors for DNA sample heating and accurate thermal cycling for DNA amplification, check valves provided in said micro-fluidic channels for controlling flow of said sample into said chamber and reaction products out of said chamber, a thermal pathway placed below and around the said reaction chamber for conducting heat from said reaction chamber, a heat sink placed below said thermal pathway covering the entire thermal pathway to facilitate fast cooling of said sample.