BigPatents India

The present invention relates to a novel method for synthesizing peptides in solution, the method comprising: a) treating a peptide or amino aciGGHULYDWLYHZKLFKKDVDQ.-amine function blocked with a urethane group of the formula R1-SO2-CH2CH2-O-CO-, wherein R1LVDU\OZLWKDQH[FHVVRIDQDOLSKDWLFVHFRQGDU\DPLQHLQDQRUJDQLFVROYHQWWRSURYLGHWKHUHOHDVHRIWKHIUHH.-amine function of said amino acid or peptide derivative and the formation of a tertiary amine adduct between said secondary amine and the liberated vinyl compound of the formula R1-SO2-CH2=CH2; b) removing the solvent and the excess of said secondary amine; c) contacting thus formed mixture of the N.-deprotected amino acid or peptide derivative and said tertiary amine adduct with subsequent peptide or amino acid derivative N.-protected with the above indicated or another urethane group under conditions providing the formation of a peptide bond betweeQWKHIUHH.-amine function of the N.-GHSURWHFWHGDPLQRDFLGRUSHSWLGHGHULYDWLYHDQGDQ.-carboxylic function of the subsequent N.-protected peptide or amino acid derivative; d) separating the newly formed peptide from the reaction mixture; e) repeating procedures as set forth above until the desired polypeptide is obtained. (FIG.NIL )